How Our Technology Works

Aperiomics solves inadequate pathogen testing by combining deep next-generation sequencing (NGS) with its powerful Xplore-PATHO℠ software.  This combination of technologies allows virtually any biological sample to be tested for virtually any microorganism.

Solution Overview:

  1. Collect sample (swab, blood, tissue, urine, stool, plant, animal, etc.)
  2. Extract genetic information (DNA and/or RNA) from sample in CLIA/CAP laboratory
  3. Use NGS to generate raw sequence data from all microorganisms in sample
  4. QC and analyze raw sequence data using Xplore-PATHO℠ to identify pathogens in sample
  5. Report all bacteria, DNA viruses, parasites and fungi identified along with the abundance of each
Fact Sheet
Sample Guide

Finally, a pathogen identification tool that works! Aperiomics gave us the actionable starting point to get our patient on a proper treatment plan.

Guadalupe Zamora, MD Family Medicine

Aperiomics has successfully found many infections missed by standard testing. By utilizing the Aperiomics Microbial Database, my interstitial cystitis patients have been able to finally identify not only bacteria, but fungal and viral infections contributing to their chronic bladder symptoms and then receive the appropriate treatment needed to cure what was previously thought to be a chronic, degenerative, and incurable condition.

Ruth Kriz, APRN

Getting Started

Set up free account

Order collection kits to have on hand

Collect patient samples

Complete requisition form

Ship to Aperiomics at no cost

Receive & interpret report

Superior, Cutting-Edge Technology

Aperiomics uses the latest technology from the best minds in genomics and microbial testing. Next-generation sequencing is performed using Illumina sequencers. Once a complete genetic fingerprint of the sample is created, the human portion is removed and the remaining microbial data is run through our proprietary software and against the Aperiomics Microbial Database™. It’s a comprehensive database of microorganisms, including the world’s largest collection of pathogens. This technology allows “all-in-one” testing from any sample and is only available through Aperiomics.

Clinician FAQs

What sample kits do you offer?2019-04-25T15:05:00-04:00

KITS OFFERED: blood plasma, fecal (feces), urine, tissue, swab, sputum (deep throat saliva and mucus), cerebrospinal fluid, prostate secretion, and breast milk.

*swabs can also be used for environmental tests, such as your home, floors, outside surfaces, etc. 

Why do I need a physician in order to get this test?2019-02-20T21:47:40-04:00

In the US, it is required to have a physician to support you in order to have this test.

The physician will provide ICD-10 codes for potential insurance reimbursement as well as provide result analysis in order to diagnose and create a treatment plan to get you better.

Do I need a supporting doctor to able to order this test?2019-02-20T21:45:56-04:00

US patients require a supporting physician in order to get this test. We cannot send you a test until you have a doctor who is willing to support you by reviewing your results and creating a treatment plan post result review.

If needed, your physician can schedule a consultation with our infectious disease doctor on staff.

Do you ship kits/ serve patients internationally?2019-02-25T18:49:04-04:00


We serve patients in most major international areas, and do this often.


Which sample kit should I order?2019-04-29T16:01:28-04:00

KITS OFFERED: blood plasma, fecal (feces), urine, tissue, swab, sputum (deep throat saliva and mucus), cerebrospinal fluid, prostate secretion, and breast milk.

*swabs can also be used for environmental tests, such as your home, floors, outside surfaces, etc. 

For most accurate results, we test from the closest source of infection(s), where you feel your symptoms. For example, if you have an upset stomach or diarrhea, we would have you do a fecal kit, or for urinary tract infections, we would suggest a urine kit, etc.

US patients require a supporting physician in order to receive the test. Typically, your physician can identify which test kit you will need.


Who is Aperiomics?2019-02-20T21:17:14-04:00

Aperiomics is the only company of its kind and scope in the world. It is revolutionizing the way that medical professionals around the world detect and diagnose infections. Supported by the National Science Foundation, Aperiomics identifies every known bacteria, virus, fungus, and parasite through deep shotgun metagenomic sequencing. We help doctors and their patients identify the causes of infections that other tests cannot identify, thus streamlining the path to a positive clinical outcome. Many patients that we have helped were sick for years with no understanding of why. Patients and their physicians often contact Aperiomics after hearing about our life-changing success stories. The complete genetic fingerprint of a sample is created and compared to Aperiomics’ Xplore- PATHO database – a comprehensive database of over 37,000 microorganisms, including the world’s largest collection of known pathogens. Aperiomics was named Life Science’s Innovator of the Year in 2016 and International Start Up of the Year in 2018.

What does Aperiomics test for?2019-02-22T16:01:20-04:00

Aperiomics tests for all known bacteria, virus, parasites and fungi based on the sequenced genomes from thousands of scientists around the world.  This includes microbes that can cause intestinal issues, urinary tract infections, Lyme disease and many other issues.

What exactly does “parasite” mean?2019-02-22T16:11:51-04:00

We at Aperiomics define parasites as the currently sequenced parasitic worm, amoeba, and archaea genomes. Outside of worms this does not include multicellular things like mites or insects. Mites and multicellular organisms are typically glaringly obvious through a simple microscope and do not require the cutting edge DNA sequencing Aperiomics provides to diagnose.

Will Aperiomics contact clinicians?2019-02-20T21:19:02-04:00

Aperiomics will want to talk to clinicians for the first time account setups. This is a simple process and we will have someone to help them along the way.  An Aperiomics representative will call to notify the clinician’s office when the report is done, that way, you and your physician can sit down with the results and a treatment plan can be created.

Only US patients require a supporting physician.

Do patients or customers need to be sick in order to have Aperiomics test them?2019-02-20T21:20:20-04:00

No, patients or general customers do not need to be sick to be tested.  In some cases it may be desired to know one’s normal microbial population.  Aperiomics can perform a complete metagenomic microbiome assessment from any clinical sample. 

Many patient’s have found it beneficial to know what their healthy body’s microbial state looks like to serve as a baseline for comparison in the event they do become ill in the future.

Do I have to be a doctor to order your testing services?2019-02-20T21:21:06-04:00

Aperiomics typically requires a clinician be involved in the collection and ordering of our testing.  This allows for the proper documentation for potential insurance reimbursement, as well as allowing for proper interpretation of results for the patient.

International patients do not need a supporting physician to order the test.

Why would there be a need to test multiple samples at one time?2019-02-20T21:24:04-04:00

In some cases, if there is not a clear, single site of infection (or symptom), we will request that multiple samples be collected and tested.  In this case, we will request multiple sample to ensure the best possible results.

Is there anything to be careful of when shipping samples?2019-02-20T21:25:39-04:00

When collected as instructed, samples are rendered non-infectious and stable at ambient temperatures for up to 30 days.  The supplied shipping materials will return the sample next-day, and thus no special concerns are needed when shipping samples back to Aperiomics.

Your physician (for US patients only) and you will receive your results within 10-14 days after sample is returned to Aperiomics.

Are there any medications, probiotics, or treatment to discontinue before testing?2019-02-20T21:27:46-04:00

For us to get the best possible sample, it is typically recommended that all anti-infective treatments (antifungals, antiparasitics, antibiotics) be stopped 3 days prior to sample collection. This should be discussed with a healthcare provider. 

Probiotics should be stopped 14 days prior. All anti-infective treatments and probiotics should be listed on the requisition form, so our infectious disease doctor is aware of this when interpreting your results during the post- results physician consultation.

Do the biologicial samples have to be refrigerated or frozen?2018-11-08T14:18:22-04:00

No.  Our specialized collection kits contain a solution that renders samples non-infectious and keeps the genetic material that we need intact for up to 30 days at room temperature.  Combined with our supplied packing materials, it also makes it safe for shipping… so no need to worry.

How is the appropriate type of sample(s) for testing determined and what collection kits should be ordered?2019-02-20T21:30:07-04:00

Physicians should be able to tell patients which kit or kits they’ll need to help identify the pathogens causing your illness.  While a single kit may be needed, in some instances patients may require additional kits (blood, swab, urine, fecal, tissue or semen) to capture the pathogens at the suspected source of the infection. 

For most accurate results, we test from the closest source of infection(s), where you feel your symptoms. For example, if you have an upset stomach or diarrhea, we would have you do a fecal kit, or for urinary tract infections, we would suggest a urine kit, etc. If there are NO CLEAR SIGNS OF SYMPTOMS, we suggest a blood plasma kit.

How long does it take to get the test results?2019-08-13T12:55:38-04:00

Upon receipt of the patient’s sample kit, it typically takes 2-3 weeks for a report to be generated and uploaded to the clinician portal.


Once the report is generated and sent to the ordering clinician, the clinician can sign up for a complimentary consultation with one of our infectious disease experts on staff. Our on staff infectious disease expert will walk the ordering clinician through the report and provide them with insight, context, and understanding surrounding the report.


When the ordering clinician wishes to schedule a complimentary consultation, they can do so on their clinician portal.

How do I get the results?2019-02-20T21:40:41-04:00

Results are sent to healthcare providers securely and directly. For international patients, results are sent to you or your physician on file (if applicable).

If needed, one can schedule a consultation with our infectious disease doctor on staff to interpret and understand patient results.

How does Aperiomics testing differ from what other companies offer?2018-11-08T14:31:59-04:00

Most labs test for just one or a handful of pathogens at a time. Others are only focuse on one aspect like bacteria.  Aperiomics tests for ALL known pathogens at once!  This is the most comprehensive testing available on the market and one of the best options for identifying chronic or cryptic infections.

Why is next-generation sequencing(shotgun metagenomics) testing more accurate than culture-growing testing?2018-11-08T14:45:04-04:00

Culture is a 100+ year old technology that is limited to identifying only what will grow in culture.  Given that many microorganisms will not grow in culture, this technology is dramatically limited in the number of microorganisms that can be identified.  Culture creates bias because just one bacterial cell can grow and render a sample positive for that bacteria, irrespective of if that bacteria is causing an active infection.  Additionally, culture does not allow for consistent, objective identification of microorganisms to the species level.

Not all NGS techniques are the same though, there are multiple different forms. Some, used by our competitors only look at bacteria and have difficulty looking at the species and strain level. The type of NGS we use, called shotgun metagenomic sequencing, is one of the most powerful among NGS techniques able to do it all.

Shotgun metagenomics is more specific than culture can be because it identifies the identity of microorganisms according to the genetic sequence and not simply appearance of growth in culture.

What’s the difference between PCR and next-generation sequencing testing?2018-11-08T15:00:21-04:00

PCR is a technology that makes many copies of a small portion of a microorganism’s genetic information.  It can be inexpensive and sensitive, but is extremely limited in how many microorganisms can be identified in a single test – typically less than a dozen in one test.  Further, because PCR creates many copies of small genetic pieces, it is heavily biased and cannot be used to assess abundance of microbial populations.


In contrast, NGS  (shotgun metagenomic sequencing) creates a genetic fingerprint of everything in the sample.  It is a much more comprehensive approach, capable of identifying tens of thousands of microorganisms in one sample, only limited by the availability of sequence data.  Aperiomics’ approach to NGS reduces as much bias as possible and allows abundance of microbial populations to be assessed.

What’s the difference between 16S NGS and shotgun metagenomics sequencing NGS?2018-11-08T15:12:26-04:00

16S NGS is similar to PCR in that it creates many copies of specific genetic information.  These copies are then compared to a database to identify the bacteria present in a given sample.  16S sequencing is good at identifying thousands of bacteria to the genus level, but is unable to identify virus, fungi, or parasites.  Deep shotgun metagenomics sequencing, on the other hand, is able to identify tens of thousands of microorganisms and differentiate species of bacteria, virus, parasites, and fungi.

What does Aperiomics have planned in the future?2017-07-10T19:56:41-04:00

Items that are in the pipeline or in development now include drug resistance identification, the ability to identify true unknowns, disease prediction and biosurveillance.


How can Aperiomics services with our comprehensive list of known pathogens help with your testing needs?